Blood status analysis is a standard read-out performed during the whole blood loop assay with the use of hematology analyzer XP-300 (Sysmex), a proven technology for accuracy of results.
Eight parameters are analyzed: platelet (PLT) count, white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). WBCs, RBCs and PLTs are counted using the direct current detection method with coincidence correction. Automatic discriminators separate the cell populations based on complex algorithms. The intensity of the electronic pulse from each analyzed cell is proportional to the cell volume. The hematocrit (HCT) is directly determined based on the red cell count and volume detection of each individual RBC. Hemoglobin analysis is conducted using a non-cyanide method.