Advanced Characterization Services for Biologicals and other Therapeutics
The use of the human whole blood loop system to investigate differences in effector mechanisms of a therapeutic antibody in whole blood versus local tissue compartments:
Antibody induced CD4 down-modulation of T cells is site-specifically mediated by CD64+ cells. Vogel S, Grabski E, Buschjäger D, Klawonn F, Döring M, Wang J, Fletcher E, Bechmann I, Witte T, Durisin M, Schraven B, Mangsbo SM, Schönfeld K, Czeloth N, Kalinke U. Scientific Reports 5, 2015 Download the article on nature.com
The use of the human whole blood loop system as a means to investigate how a nucleic acid based immunotherapeutic interacts with complement:
Complement activation by CpG in a human whole blood loop system: mechanisms and immunomodulatory effects. Mangsbo SM, Sanchez J, Anger K, Lambris JD, Ekdahl KN, Loskog AS, Nilsson B, Tötterman TH. J Immunol. 2009 Nov 15;183(10):6724-32.
Link pubmed: http://www.ncbi.nlm.nih.gov/pubmed/19864604
Therapeutic Vaccine Development
PoC article, circulating antibodies facilitates cross-priming of antigens. The findings herein implicate that the therapeutic effect of a protein/ peptide-based vaccine can be boosted if delivered in an immune complex formula:
Circulating specific antibodies enhance systemic cross-priming by delivery of complexed antigen to dendritic cells in vivo. van Montfoort N, Mangsbo SM, Camps MG, van Maren WW, Verhaart IE, Waisman A, Drijfhout JW, Melief CJ, Verbeek JS, Ossendorp F. Eur J Immunol. 2012 Mar;42(3):598-606.
Link pubmed: http://www.ncbi.nlm.nih.gov/pubmed/22488363
Using antibodies as shield to protect vaccine components can prolong antigen-presentation and thereby the effect of the vaccine:
Antigen storage compartments in mature dendritic cells facilitate prolonged cytotoxic T lymphocyte cross-priming capacity. van Montfoort N, Camps MG, Khan S, Filippov DV, Weterings JJ, Griffith JM, Geuze HJ, van Hall T, Verbeek JS, Melief CJ, Ossendorp F. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6730-5.
Link pubmed: http://www.ncbi.nlm.nih.gov/pubmed/19346487