White Blood Cell Activation & Depletion

Advanced flow cytometry-based assays of all major white blood cell populations.

Leverage the benefits of our whole blood assays

T cells

T cells are central mediators of adaptive immunity. We assess T cell activation and depletion as well as cytokine release.

B cells

B cells play important roles in the adaptive immune response. We assess B cell activation and depletion by flow cytometry.

NK cells

NK cells play important roles in both innate and adaptive immunity and are the main effector cells in ADCC. We investigate NK cell activation, depletion, degranulation and depletion of target cells.

Monocytes

Activated monocytes promote cytotoxicity and release pro-inflammatory cytokines. Monocyte activation is assessed by flow cytometry and cytokine release assays.

Granulocytes

Neutrophils, basophils, and eosinophils activation results in granule release, which can cause allergic reactions, tissue damage, and inflammation. We identify counts and activation status and analyse cytokine release.

Cell Activation

Accurately predict effects on white blood cells in human whole blood

To assess whether a therapeutic agent induces activation or depletion of the various white blood cell types, fresh human whole blood is incubated with test substances in ID.Flow. Our custom flow cytometry panel is designed to detect changes in cell activation and quantify any reduction in cell populations of cells. Whole blood samples can be analysed at multiple time points to track changes over time. Scroll down for data examples of various cell types incubated in ID.Flow and analysed by flow cytometry.

  • B cells (CD19+, CD69)
  • T cells (CD3+, CD69)
  • NK cells (CD56+, CD3–, CD69, CD107a)
  • Monocytes (CD14+, CD66b–, CD83)
  • Granulocytes:
    • Eosinophils (CD66b+, CD16–, CD69, CD11bact)
    • Basophils (SSClow, CD123+, HLA-DR–, CD203c, CD63)
    • Neutrophils (CD66b+,CD16+, CD11bact)

 

ID.Flow and flow cytometry

Assessment of white blood cells

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Comprehensive immune-assessment – interconnected readouts

Immuneed offers comprehensive services to assess the safety of biologics, small molecules and oligonucleotides. All our services are based on our innovative test system ID.Flow. The ID.Flow is capable of assessing over 40 readouts from every individual whole blood sample and it produces a wide range of data for each test item. Besides assessing WBCs, we can simultaneously assess:

  • Platelets
  • Cytokine activation
  • Complement activation
  • ADCC & CDC
  • Haematology

Immuneed conducts all studies in-house, managing every step from study design and blood donor recruitment to lab work and data analysis. Our Scientific Advisors interpret results and create flexible, project-specific reports tailored to meet each client’s unique needs. Get reliable, in-house immunological assessments tailored to your project’s needs. Contact Immuneed to discuss your study design, donor recruitment, and customized reporting options for precise, actionable results

OTHER READOUTS

Cytokine Release

Sensitive cytokine release prediction in fresh human whole blood.

Complement Assays

Assessment of C3a, C5a, and Bb split product in fresh human whole blood with active complement.

Platelets, Count & Activation

Platelet activation, conjugates, aggregation, counts, inhibition...

ADCC / CDC

Simultaneous study of ADCC and CDC in fresh human whole blood.