Leverage the benefits of the unique test system ID.Flow®
Physiologically Relevant
Our assay, ID.Flow mirrors the in vivo blood compartment, ensuring that key immune cell responses are effectively captured.
Superior Sensitivity
ID.Flow has a superior ability to detect and grade immune responses compared to animals and cell based assays such as PBMCs.
Expert-Led Interpretation
Our team of immunology experts provides in-depth data interpretation and flexible report formats: from Excel to IND-compatible reports.
CYTOKINE ASSAY
Why choose our cytokine assay?
- Whether working with oligonucleotides, antibodies, or other biologics, our assay ensures relevant data for your therapeutic class.
- By assessing cytokine release in fresh human whole blood, ID.Flow detects immune responses with better precision than plate-based assays.
- Regulatory Compliance: Our assay adheres to the latest FDA and EMA guidelines. It is classified as a New Approach Method, which is encouraged by the FDA
ID.Flow exhibits minimal background and significantly less donor variability.
The figures above shows a comparison between ID.Flow and two plate-based assays that used either PBMCs with coated antibodies or whole blood with soluble antibodies to assess cytokine release. The antibodies used in the tests were classified as having a low risk of causing CRS, such as anti-integrin alpha 4 (1 µg/ml) and anti-EGFR antibody (200 µg/ml), and high risk of causing CRS, such as anti-CD52 antibody (3 µg/ml) and anti-CD28 (aCD28). The level of cytokines was compared to the vehicle and zero (blood directly after acquisition). Readouts from ID.Flow were taken after 4 hours which mirrors the actual time for cytokine release upon IV-administration in humans, whereas the plate-based assays required a 24-hour incubation period to distinguish a positive response from a negative.
The data from the comparative study demonstrate that ID.Flow has a superior ability to detect cytokine release. This is shown by the greater signal-to-noise ratio, i.e. greater separation of cytokine levels induced by the high risk antibodies than the low risk antibodies , which makes it possible to detect not only super-agonistic responses but also low-to-moderate responses.
ID.Flow cytokine assessment
- The freshly donated human blood is immediately transferred to the ID.Flow test system, followed by administration of the test items, and set to circulate at 37°C to prevent clotting.
- Samples are collected at baseline and selected timepoints, then processed to plasma by centrifugation.
- Cytokine analysis is conducted on the Multi-Array platform from Meso Scale Discovery (MSD).
Readouts
- The standard proinflammatory cytokine panel includes cytokines of the adaptive and the innate arm: IFN-γ, IL-2, IL-6, IL-8 and TNF-α.
- All available analytes for the MSD platform can be included in the study.
Read more about how ID.Flow was used to test a TGN1412-like antibody, Fletcher Int Immunopharmacol. 2017 Oct 27;54:1-11. follow the link. (ID.Flow = whole blood loop system in this publication)
ID.Flow offers a range of additional readouts for every test condition.
Complement Assays
Assessment of C3a, C5a, and Bb split product in fresh human whole blood with active complement.
White Blood Cells, Count & Activation
Activation of B cells, T cells, NK cells, monocytes, and granulocytes is simultaneously analyzed in fresh human whole blood.
Platelets, Count & Activation
Platelet activation, conjugates, aggregation, counts, inhibition...
ADCC / CDC
Simultaneous study of ADCC and CDC in fresh human whole blood.