Customer promise
QUALITY
Ex-vivo assessment that helps you understand your drug’s potential to mediate cytokine release and cell activation. Our cytokine assay offers unmatched sensitivity in evaluating the potential for adverse pro-inflammatory reactions.
RELEVANCE
ID.Flow provides a unique and physiologically relevant testing environment. Using circulating whole blood, we detect responses frequently missed out by plate-based assays.
FLEXIBILITY
Immuneed's Scientific Advisors will design the study to suit your unique project. Choose between a scientific reporting format that suits your project: Standard Report IND-enabling Report
Cytokine Assay
Cytokine release – a critical aspect of preclinical safety assessment.
Regulatory authorities demand an assessment of cytokine release potential, emphasizing the importance of human-based methods in determining potential immunotoxicity. Leverage the benefits of CRA in Immuneed’s innovative platform, ID.Flow. It offers a sensitive prediction of cytokine release in an environment that closely mimics human blood circulation.
Traditional plate-based assays require high levels of anticoagulants which increase the risk of a missed response by inactivating complement and cascade systems. In contrast, ID.Flow utilizes fresh whole blood with all cells and systems present and active. ID.Flow can provide readouts within minutes to hours. This perfectly mirrors the clinical situation, where reactions develop swiftly after drug administration and not following a lengthy incubation period. With the high sensitivity and speed of our method, you can effectively assess the hazards of cell activation and cytokine release posed by your drug candidate.
Scroll down to contact us for a first meeting. Together with our Scientific Advisors, we’ll design the study to address your specific needs. Our Scientific Reports are flexible, choose between a standard report or an IND-enabling report.
The analytic power of ID.Flow
Shown above is a direct comparison of Immuneed’s platform, ID.Flow with two plate-based assays where either PBMCs with coated antibodies or whole blood with soluble antibodies were used for cytokine release assessment. It involves antibodies associated with a low risk of causing CRS, namely anti-integrin alpha 4 (1 µg/ml) and anti-EGFR antibody (200 µg/ml), as well antibodies associated with a high risk of CRS, namely anti-CD52 antibody (3 µg/ml) and anti-CD28 (aCD28). The level of cytokines as compared to vehicle and zero (blood directly after the acquisition). In the ID.Flow, cytokine release was measured at a time point of 4 hours, while the plate-based assays required a 24-hour incubation time before readouts could be taken.
Read more about when Immuneed’s platform was used to test a TGN1412-like antibody, Fletcher Int Immunopharmacol. 2017 Oct 27;54:1-11. follow the link. ID.Flow is called whole blood loop system in this publication.
Analysis by ID.Flow
Your drug is infused into fresh human blood in circulation.
Read-outs are taken to perfectly mimic the clinical situation of infusion reactions.
The multiplexing platform from MesoScale Discovery (MSD) is used for analyzing the samples.
All available analytes for the MSD platform can be included in the cytokine evaluation if you wish.
Readouts
The standard proinflammatory cytokine panel includes cytokines of the adaptive and the innate arm: IFN-γ, IL-2, IL-6, IL-8 and TNF-α. Other readouts are also available upon request.
OTHER SERVICES

Complement activation and inhibition
Simultaneous study of all complement responses - in one assay.

Cell binding & activation
Analysis of all cells in the human circulation - in one assay.

Antibody-Dependent Cell-Mediated Cytotoxicity and Complement Dependent Cytotoxicity
Simultaneous study of ADCC and CDC offers several advantages - and is unique in preclinical testing.

Monocyte activation
Detect any potential drug impurities and understand the safety profile of your drug.