Leverage the benefits of our whole blood assays
T cells
T cells are central mediators of adaptive immunity. We assess T cell activation and depletion as well as cytokine release.
B cells
B cells play important roles in the adaptive immune response. We assess B cell activation and depletion by flow cytometry.
NK cells
NK cells play important roles in both innate and adaptive immunity and are the main effector cells in ADCC. We investigate NK cell activation, depletion, degranulation and depletion of target cells.
Monocytes
Activated monocytes promote cytotoxicity and release pro-inflammatory cytokines. Monocyte activation is assessed by flow cytometry and cytokine release assays.
Granulocytes
Neutrophils, basophils, and eosinophils activation results in granule release, which can cause allergic reactions, tissue damage, and inflammation. We identify counts and activation status and analyse cytokine release.
Cell Activation
Accurately predict effects on white blood cells in human whole blood
To assess whether a therapeutic agent induces activation or depletion of the various white blood cell types, fresh human whole blood is incubated with test substances in ID.Flow. Our custom flow cytometry panel is designed to detect changes in cell activation and quantify any reduction in cell populations of cells. Whole blood samples can be analysed at multiple time points to track changes over time. Scroll down for data examples of various cell types incubated in ID.Flow and analysed by flow cytometry.
- B cells (CD19+, CD69)
- T cells (CD3+, CD69)
- NK cells (CD56+, CD3–, CD69, CD107a)
- Monocytes (CD14+, CD66b–, CD83)
- Granulocytes:
- Eosinophils (CD66b+, CD16–, CD69, CD11bact)
- Basophils (SSClow, CD123+, HLA-DR–, CD203c, CD63)
- Neutrophils (CD66b+,CD16+, CD11bact)