Cytokine Release Assay

Assessment in circulating human whole blood.

Leverage the benefits of the unique test system ID.Flow®

RELEVANCE

ID.Flow mirrors the intravascular environment ensuring that key immune responses are assessed in a physiologically relevant environment.

SENSITIVITY

ID.Flow has a superior ability to detect and grade immune responses compared to animals and cell based in-vitro assays.

CYTOKINES

Our standard panel includes IL-2, IL-6, IL-8, TNFa and IFNg. Any cytokine available in the Mesoscale platform can be evaluated.

CYTOKINE ASSAY

Why choose our cytokine assay?

  • Our cytokine assay provides human-relevant data, whether you are working with oligonucleotides, biologics, or small molecules.
  • With direct access to blood donors, we evaluate cytokine responses in fresh human whole blood, ensuring precise detection.
  • ID.Flow aligns with FDA and EMA guidelines as a New Approach Method (NAM) and a Non-animal Method.

Cytokines are analysed within a test environment that preserves the complete network of plasma proteins, white blood cells, red blood cells, and platelets. This approach enables precise tracking of cytokine levels over time. This approach enables precise tracking of cytokine levels over time, alongside a broad spectrum of related immune parameters, providing a comprehensive assessment of immune activation potential within a single experiment.

Cytokine Release Assessment in ID.Flow

Graphs

Get in touch with us!

Have a project or a question, or would you like to request a quote? We'd be happy to hear from you.

Assay comparison: cytokine release.

We evaluated the predictive value of different cytokine release methods. Download our white paper to learn about the pros and cons of three different methods of assessing cytokine release.

The ID.Flow System: Extensive Immune Readouts

Immuneed offers comprehensive services to assess immunopharmacology and immunotoxicology. ID.Flow can be used to generate samples from multiple time points, allowing for the assessment of over 40 readouts from every test item. Besides analysing the cytokine release potential, we can simultaneously assess effects on:

  • Platelets
  • White blood cells
  • Complement activation
  • ADCC & CDC
  • Haematology

 

Immuneed conducts all studies in-house, managing every step from study design and blood donor recruitment to lab work and data analysis. Our Scientific Advisors interpret results and create flexible, project-specific reports tailored to meet each client’s unique needs.

Get reliable, in-house immunological assessments tailored to your project’s needs. Contact Immuneed to discuss your study design, donor recruitment, and customized reporting options for precise, actionable results

ID.Flow offers a range of additional readouts for every test condition.

Complement Assays

Assessment of C3a, C5a, and Bb split product in fresh human whole blood with active complement.

White Blood Cells, Activation & Depletion

Activation of B cells, T cells, NK cells, monocytes, and granulocytes is simultaneously analyzed in fresh human whole blood.

Platelets, Count & Activation

Platelet activation, conjugates, aggregation, counts, inhibition...

ADCC / CDC

Simultaneous study of ADCC and CDC in fresh human whole blood.